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NICU/Pediatrics Minimum Volume Requirements

Specimen Collection Microtainer® tubes (pediatric bullet tubes)*

Microtainer® tube Gold Microtainer<sup>®</sup>; Red Microtainer<sup>®</sup>; Light Green Microtainer<sup>®</sup>; Lavender Microtainer<sup>®</sup>; Small Green
Color Gold Red Light Green (amber or clear) Lavender Light Green (without gel barrier)
Additive No additive with gel barrier No additive Lithium Heparin with gel barrier K2EDTA Lithium Heparin without gel barrier
Volume 0.5 mL 0.5 mL 0.5 mL 0.5 mL .04 mL

  *Microtainer is a registered trademark of Becton, Dickinson and Company

NICU/Peds Minimum Specimen Requirements

TestmL whole blood in Microtainer® unless otherwise specified
17-OH Progesterone X17OHP

0.8 mL red Microtainer® tube - collect 2 red Microtainer® tubes or 0.8 mL in a red top tube

Sendout test: TAT: 1-4 days

7-Dehydrocholesterol
(aka Smith-Lemli-Opitz Screen)

1.2 mL (no repeats) light green Microtainer® tube - collect 3 light green Microtainer® tubes

Patient preparation: Draw blood prior to feeding.

Sendout test: TAT: 3-9 days
ACTH 1.0 mL lavender Microtainer® tube ON ICE - collect 2 Microtainer® tubes
Acylcarnitine

0.36 mL (no repeats) light green Microtainer® tube - collect 1 light green Microtainer® tube or 0.36 mL in a dark green sodium heparin tube

Patient preparation: Draw blood prior to feeding or meal.

Sendout test: TAT: 2-5 days
Albumin 0.4 mL light green Microtainer® tube - collect 1 Microtainer® tube
Aldosterone

1.2 mL gold Microtainer® tube - collect 3 gold Microtainer® tubes or 1.2 mL in a gold top tube

Patient preparation: If upright specimen is collected, it is preferable to draw blood after patient is upright for at least 2 hours.

Sendout test: TAT: 1-2 days

Alkaline Phosphatase 0.4 mL light green Microtainer® tube - collect 1 Microtainer® tube

Amino Acids, Blood XAMACP

0.3 mL in llight or dark green Microtainer® tube - collect 1 Microtainer® tube

Patient preparation: Draw blood prior to feeding or 2-3 hours after a meal.

CRITICAL FROZEN: Deliver to lab immediately

Sendout test: TAT: 2-5 days

Please submit history form with specimen: http://ltd.aruplab.com/Tests/Pdf/16

Amino Acids, Urine

3 mL random urine. First morning urine is preferred.

CRITICAL FROZEN: Deliver to lab immediately

Sendout test: TAT: 3-7 days

Please submit history form with specimen: http://ltd.aruplab.com/Tests/Pdf/16
Ammonia 0.4 mL light green Microtainer® tube ON ICE - collect 1 Microtainer® tube
Androstenedione XANDRO

0.8 mL gold Microtainer® tube - collect 2 gold Microtainer® tubes or 0.8 mL in a gold top tube

Sendout test: TAT 1-4 days
Aneuploidy panel by FISH
(aka Newborn Fish panel)

0.5 mL dark green (sodium heparin) Microtainer® tube - collect 1 dark green Microtainer® tube

Sendout test: TAT: 1-3 days Monday-Friday before 2 p.m.

BNP (B-Type Natriuretic Peptide)

0.5 mL lavender Microtainer® tube - collect 1 Microtainer® tube

Bilirubin, Micro 0.4 mL light green, amber Microtainer® tube; gold ok - collect 1 Microtainer® tube
Blood Culture 0.5 to 1 mL per protocol
Basic Metabolic Panel 0.7 mL light green Microtainer® tube - collect 2 Microtainer® tubes
BMP, bili, triglyceride, MG 0.7 mL LG – collect 2 Microtainer® tubes
Carnitine Free & Total XCARN

0.4 mL light green or dark green - collect 1 green Microtainer® tube

CRITICAL FROZEN: Deliver to lab immediately.

Sendout test: TAT: 1-4 days.

MUST submit history form with specimen: http://ltd.aruplab.com/Tests/Pdf/16

CBC 0.5 mL lavender Microtainer® tube - collect 1 Microtainer® tube
Chromosome

2 mL light green (lithium heparin, no gel barrier) Microtainer® tube - collect 4 to 5 light green Microtainer® tubes or 2 mL in a dark green sodium heparin tube

Sendout: TAT: 7-14 days
Chromosome Analysis XRTC

0.5 mL dark green (sodium heparin) Microtainer® tube - collect 2 dark green Microtainer® tubes

Sendout test: TAT: 3-10 days Monday-Friday before 2 p.m.

MUST submit history form with specimen: http://ltd.aruplab.com/Tests/Pdf/20

Chromosome + FISH

2 mL light green (lithium heparin, no gel barrier) Microtainer® tube - collect 4 to 5 light green Microtainer® tubes or 2 mL in a dark green sodium heparin tube

Sendout: TAT: 7-14 days
Comprehensive Metabolic Panel 0.8mL light green Microtainer® tube - collect 2 Microtainer® tubes
Copper 1.7 mL red or gold Microtainer® tube - collect 4 Microtainer® tubes OR 1.7 mL plain royal-blue-top tube
Cortisol 0.8 mL red, gold, or light green Microtainer® tube - collect 2 Microtainer® tubes
CMV DNA PCR Qualitative

Blood: 1.5 mL lavender Microtainer® tube - collect 3 lavender Microtainer® tubes or 1.5 mL in a purple top EDTA tube

Urine: 1.5 mL random

Sendout: TAT: 1-3 days
CMV (Cytomegalovirus) IgG & IgM 0.5 mL gold Microtainer® tube - collect 1 Microtainer® tube
CPK 0.4 mL light green Microtainer® tube - collect 1 Microtainer® tube
CRP 0.4 mL light green Microtainer® tube - collect 1 Microtainer® tube
Dehyroepiandrosterone (DHEA) XDHEA

0.8 mL gold or red Microtainer® tube - collect 2 gold or red Microtainer® tubes or 0.8 mL in a gold or red top tube

Sendout test: TAT: 1-4 days

DIC Screen

0.5 mL in lavender Microtainer® tube AND one of the following:

Two 1.0 mL light blue top Microtainer® tubes, if heparin is infusing in the line from which blood is drawn.

One 1.0 mL light blue top Microtainer® tube if drawn from a line without heparin.

Light blue Microtainer® tube must contain 0.9 mL of blood (to the black marker line just above the label on the tube).

Samples must be venipuncture or arterial line/puncture; no heelstick samples.

Light blue Microtainer® tube can be obtained from Hematology at ext. 5090.

Dihydrotestosterone XDHT

1.4 mL gold or red Microtainer® tube - collect 3 gold or red Microtainer® tubes or 1.4 mL in a gold or red top tube

Sendout test: TAT: 1- 4 days
Direct Coombs (Direct Antiglobulin Test) & Indirect Coombs 1.5 mL pink-top or lavender-top tube
Electrolyte Panel 0.6 mL light green Microtainer® tube - collect 2 Microtainer® tubes
FSH (Follicle Stimulating Hormone) 0.8 mL red Microtainer® tube - collect 2 Microtainer® tubes
Ferritin 0.8 mL red, gold, or light green Microtainer® tube (lithium heparin) - collect 2 Microtainer® tubes
Glucose 0.5 mL light green Microtainer® tube - collect 1 Microtainer® tube
Hemoglobin & Hematocrit 0.5 mL lavender Microtainer® tube - collect 1 Microtainer® tube
Hepatic panel 0.7 mL LG - collect 2 Microtainer® tubes
HIV 1 DNA PCR Qualitative ZHIVQ

1.2 mL (no repeats) lavender Microtainer® tube - collect 3 lavender Microtainer® tubes or 1.2 mL in a purple EDTA tube

Sendout test: TAT: 1-4 days
Ionized Calcium 0.4 mL light green Microtainer® tube ON ICE - collect 1 Microtainer® tube
Lactate 0.5 mL grey-top tube ON ICE
LH (Luteinizing Hormone) 0.8 mL red Microtainer® tube - collect 2 Microtainer® tubes
Lipase 0.4 mL light green Microtainer® tube - collect 1 Microtainer® tube
Liver Panel (Hepatic Function Panel) 0.6 mL light green Microtainer® tube - collect 2 Microtainer® tubes
Magnesium 0.4 mL light green Microtainer® tube - collect 1 Microtainer® tube
Microarray XSNPM

1 mL dark green (sodium heparin) or lavender (EDTA) Microtainer® tube - collect 2 dark green/lavender Microtainer® tubes

Sendout test: TAT: 3-10 days Monday-Friday before 2 p.m.

MUST submit history form with specimen: http://ltd.aruplab.com/Tests/Pdf/76

Osmolality, Serum 0.3 mL gold Microtainer® tube - collect 1 Microtainer® tube
Phosphorus 0.4 mL light green Microtainer® tube - collect 1 Microtainer® tube
Phos, Alk Phos, BMP 0.7 mL LG - collect 2 Microtainer® tubes
Progesterone 0.8 mL red Microtainer® tube - collect 2 Microtainer® tubes
Prolactin 0.8 mL red Microtainer® tube - collect 2 Microtainer® tubes
PT, PTT

Two 1.0 mL light-blue-top Microtainer® tubes, if heparin is infusing in the line from which blood is drawn.

One 1.0 mL lgiht blue top Microtainer® tube, if drawn from a line without heparin.

Light blue Microtainer® tube must contain 0.9mL of blood (to the black marker line just above the label on the tube).

Samples must be venipuncture or arterial line/puncture; no heelstick samples.

Light blue top Microtainer® tube can be obtained from Hematology at ext. 5090.

Pyruvate

Patient to be fasting and resting. Specimen must arrive within 2 hours of draw. Require the use of specimen collection tubes (provided by UCI Lab) for blood pyruvate tests. These special tubes contain a pre-measured amount of 8% perchloric acid (4.0 mL for adults and 2.0 mL for pediatrics) to which the blood will be directly added at bedside. Quality specimens and reliable results are dependent upon carefully following the procedures for blood draw and processing. Specimen collection and processing instructions:

Blood Draw:

1.  Have a small bag of ice and special orange screw top tube available prior to drawing the blood.

2.  Draw enough blood in a syringe to add 2.0 mL (1.0 mL pediatrics) to the special tube.

3.  Immediately transfer blood to the special tube:

       a. Adult tubes: add exactly 2.0 mL by filling to the 6.0 mL line.

       b. Pediatric tubes: add exactly 1.0 mL by filling to the 3.0 mL line.

4.  Invert tubes 3 times.

5.  Label the tube with proper patient identification.

6.  Place on ice.

7. Take specimen to the lab immediately.

Note: Send to lab the Pyruvate tube in a slurry of ice immediately.

Renin Activity

1 mL lavender or pink Microtainer® tube - collect 2 lavender/pink Microtainer® tubes or 1 mL in a lavender/pink EDTA tube

Patient preparation: If upright specimen is collected, it is preferable to draw blood after patient is upright for at least 2 hours. Draw blood before noon.

Sendout test: TAT: 1-2 days.

CRITICAL FROZEN: Send to lab immediately after collection.

Reticulocyte Count 0.5 mL lavender Microtainer® tube - collect 1 Microtainer® tube
Reveal SNP Microarray

2.0 mL in a dark green sodium heparin tube (minimum 1.0 mL)

Sendout: TAT 8 - 11 days

Must include integrated genetics reveal SNP Microarray Pediatric Questionnaire (on website)

SGOT 0.4 mL light green Microtainer® tube - collect 1 Microtainer® tube
SGPT 0.4 mL light green Microtainer® tube - collect 1 Microtainer® tube
Testosterone Free & Total XTESTF

1.8 mL gold or green (sodium or lithium heparin) Microtainer® tube - collect 4 Microtainer® tubes

Sendout test: TAT: 1-4 days

Testosterone Free XFTESF

1.8 mL gold or green (sodium or lithium heparin) Microtainer® tube - collect 4 Microtainer® tubes

Sendout test: TAT: 1-4 days

Thyroid FunctionTSH, T4, T3

0.8 mL red Microtainer® tube for any single test - collect 2 Microtainer® tubes

1.5 mL red Microtainer® tube for any two or three tests - collect 3 Microtainer® tubes

If Hct < 45%: 1.0 mL red Microtainer® tube - collect 2 red Microtainer® tubes or 1.0 mL in a red top tube - for any 2 or 3 tests
Thyroxine (T4) Free 0.8 mL red Microtainer® tube - collect 2 Microtainer® tubes
TSH, Free T4

0.8 mL red Microtainer® tube for one test - collect 2 Microtainer® tubes

1.2 mL red Microtainer® tube for both tests - collect 3 Microtainer® tubes

If Hct < 45%: 0.8 mL red Microtainer® tube for both tests - collect 2 Microtainer® tubes
TIBC (Total Iron Binding Capacity) 0.5 mL red or gold Microtainer® tube - collect 1 Microtainer® tube
Toxoplasma IgG & IgM 0.5 mL gold Microtainer® tube - collect 1 Microtainer® tube
Triglycerides 0.4 mL light green Microtainer® tube - collect 1 Microtainer® tube
Uric Acid 0.4 mL light green Microtainer® tube - collect 1 Microtainer® tube
Urine Drug Screen RDSI: 1 mL urine
UDS: 6 mL urine
Urine Electrolytes 0.4 mL spot urine in a screw-cap tube
Urine Organic Acids Screen

3 mL urine

Sendout: TAT: 3-6 days (Not reported Saturday or Sunday)
Very Long Chain Fatty Acids ZVLCFA

0.8 mL (no repeats) red Microtainer® tube - collect 2 Microtainer® tubes

Sendout test: TAT: 4-7 days

Therapeutic Drug Levels

Amikacin 0.5 mL red Microtainer® tube - collect 1 Microtainer® tube
Caffeine 0.5 mL red Microtainer® tube - collect 1 Microtainer® tube
Digoxin 0.5 mL red Microtainer® tube - collect 1 Microtainer® tube
Dilantin 0.4 mL red Microtainer® tube - collect 1 Microtainer® tube
Gentamicin 0.4 mL red Microtainer® tube - collect 1 Microtainer® tube
Phenobarbital 0.4 mL red Microtainer® tube - collect 1 Microtainer® tube
Theophylline 0.4 mL red Microtainer® tube - collect 1 Microtainer® tube
Tobramycin 0.4 mL red Microtainer® tube - collect 1 Microtainer® tube
Vancomycin 0.4 mL red Microtainer® tube - collect 1 Microtainer® tube

Laboratory Contacts

Sr. Supervisor Jennifer Zhang 714-456-5281 zhangj1@uci.edu
AM Supervisor Katie Nguyen-Vu 714-456-8822 knguyenv@uci.edu
PM Supervisor Robyn Kanemoto 714-456-8822 rkanemot@uci.edu
Send Outs Raspinder Seewat
Danielle Howard
714-456-5470 Path-Sendout@hs.uci.edu
General Info Weekends/Holidays 714-456-5500

Updated 4/5/16